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. 2017 Dec 19;7:17785. doi: 10.1038/s41598-017-18138-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of signaling pathways targeted by miR-196a and miR-196b. Total protein lysates from UT-SCC-42B and FaDu cells transfected with either premiR-196a, premiR-196b or non-targeting control were applied to a proteome phospho-kinase array. (A) Relative signal intensity values (to control-transfected cells) are displayed as a heat map. (B) Western blot validation of the array data. (C) and (D) Quantification of IRDye fluorescent signals. *p < 0.05, **p < 0.01 and ***p < 0.001 by Student t-test.