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. 2017 Dec 19;7:17751. doi: 10.1038/s41598-017-17665-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptome profiling of HB cell lines and tumor samples. (a) PCA of RNA sequencing data of two parental HB cell lines (HepG2-C, Huh-6-C) and two cell lines grown in vivo as xenograft tumors (HepG2-T, Huh-6-T), compared with RNA sequencing data of six normal liver samples and nine primary HB tumor samples. The two HepG2 samples and the Huh-6 sample grown in vitro form a cluster, indicating that their gene expression is similar to each other. The Huh-6 tumor sample is located far from the other clusters, showing that it has more transcriptome differences from the primary tumors and other cell line and xenograft samples. (b) GSEA to reveal transcriptome differences between cells grown in vitro and in vivo as xenograft tumors. (c) Molecular clustering of HB cell lines and tumor samples. Heat map analysis of 16-gene HB signature²⁷ in nine primary HB tumors (yellow) and four HB cell line and xenograft samples (HepG2 (green), Huh-6 (blue)), normalized to six normal liver samples. The cell line and xenograft samples show the C2 signature, which is associated with a worse prognosis. (d) Spearman correlation of 18571 HUGO genes to indicate gene expression similarities of two parental cell lines and two luciferase-transduced cell lines all grown in vitro.