Fig. 1.

Screening of generic compounds to prevent iron-mediated neurotoxicity. Shown is an example of a screening of drugs to identify those that prevent iron-mediated neurotoxicity to human neurons. Neurons were pretreated with drugs at a concentration of 10 µM, followed by a challenge with 25 or 50 µM FeSO₄ after 1 h. In this experiment, several compounds (yellow bars) prevented against iron-mediated neurotoxicity as determined by the number of survival neurons in automated counts after 24 h (a). Values in a are mean ± SEM of n = 4 wells per condition. One-way analysis of variance (ANOVA) with Bonferroni post hoc analysis vs. iron: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. b–d Representative images show the untreated control and iron-treated neurons, as well as the prevention of neurotoxicity by indapamide (top: bright field; bottom: fluorescence microscopy). Neurons were detected by anti-MAP-2 antibody. The scale bars depict 100 µm