Fig. 2.

Summary of compounds that attenuate iron-mediated neurotoxicity. Shown are all 35 generic drugs that prevent iron-mediated neurotoxicity (a). The number of neurons in each well of a given experiment was normalized to the number of neurons of the respective untreated control condition (100%). The corresponding FeSO₄-treated condition (red) was also normalized to the respective control. Some of the major drug classes are depicted in the figure. Shown are the mean ± SEM of 2–4 independent experiments, performed in quadruplicates (thus, 8–16 wells per treatment across experiments are depicted in the figure). b shows the results from live-cell imaging of neurons (see examples in Supplementary Video 1), challenged with FeSO₄ in a concentration of 50 µM. Upon pre-treatment with indapamide or desipramine 1 h before the addition of iron, the number of propidium iodide-positive cells was significantly reduced after 7.5 h and even below the level of the untreated control condition after 12 h, suggesting a strong neuroprotective effect. Live-cell imaging was performed over 12 h, where images were taken every 30 min. The time-point from which significant changes from FeSO₄ were observed for each group is marked with a symbol (# control; + DMSO; * indapamide; ~ desipramine). Shown are means ± SEM of n = 3 wells per condition. Results were analyzed with a two-way ANOVA with Dunnett's multiple comparison as post hoc analysis