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Subcloning of recombinant cells for clone generation.
| Experimental No. | Parental cells (genotype) | No. of cells | No. of recombinant clones | Random integration | Unexpected mutation |
|---|---|---|---|---|---|
| 1 | 820-1 (C/C) | 1.5 × 106 | 1 | − | − |
| 820-2 (G/C) | 1.5 × 106 | 0 | |||
| 2 | 820-1 (C/C) | 2.0 × 106 | 1 | − | − |
| 820-2 (G/C) | 2.0 × 106 | 1 | − | + | |
| 3 | 820-2 (G/C) | 3.0 × 106 | 1 | + | + |
| 4 | 820-2 (G/C) | 6.0 × 106 | 0 | ||
| 5 | 820-2 (G/C) | 6.0 × 106 | 0 | + |
