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. 2017 Dec 19;8:2203. doi: 10.1038/s41467-017-02397-0

Fig. 4.

Fig. 4

Native mass spectrometry reveals the effect of lipid tail chemistry on allosteric modulation of AmtB–GlnK. a Plot of equilibrium dissociation constants (K D) for GlnK binding apo AmtB and AmtB bound to PG lipids with increasing acyl chain length: 12 (DL, 1,2-dilauroyl), 14 (DM, 1,2-dimyristoyl), and 16 (DP, 1,2-dipalmitoyl). b Plot of K D for GlnK binding apo AmtB and AmtB bound to PE lipids with different stereochemistry: dioleoyl (DO, 18:1) in cis (cis-DOPE) or trans configuration (trans-DOPE), and 1-stearoyl-2-oleoyl (SO, 18:0–18:1) in cis configuration (cis-SOPE). Reported are the average and s.e.m. from repeated measurements (n = 3)