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. 2017 Dec 19;7:17819. doi: 10.1038/s41598-017-17862-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DKK2 restores cavernous endothelial and pericyte content. (a) PECAM-1 (red) and NG2 (green) staining of cavernous tissue from sham operation group or CNI mice 1 week after receiving intracavernous injections of PBS (20 μl) or DKK2 protein (days -3 and 0; 6 µg/20 µl). Nuclei were labeled with DAPI (blue). Scale bar 100 µm (upper row), and 25 µm (lower row). (b,c) Quantitative analysis of cavernous endothelial cell and pericyte content was performed by an image analyzer. Each bar depicts the mean (±SE) values from n = 6 animals per group. *P < 0.05 vs. sham operation group, ^# P < 0.05 vs. PBS-treated CNI group. CNI = cavernous nerve injury; DKK2 = dickkopf2.