Fig. 1. The diagnosis and monitoring of CML. (A) Conventional karyotype analysis by G-banding showing typical translocation between chromosome 9 and 22 (arrows). (B) FISH analysis using BCR-ABL dual fusion probe. BCR-ABL-positive cells (red arrow), normal cells (green arrow). (C) Sanger sequencing of the nested RT-PCR product demonstrating the direct junction between BCR exon e8 and ABL exon a2 (reference sequence: BCR, NM_004327.3; ABL1, NM_005157.5). (D) Clinical response to treatment monitored by FISH.
