Fig. 3.

VEGFR1-induced cell responses are primarily directed by PLCγ, PI3K, and Src concentrations. HUVEC a–c Integrated responses (area under the activation–time curve) and d–f phosphorylation amplitudes for each cell response, stemming from VEGFR1 signaling only, were quantified with respect to all VEGFR1-associated adapter concentrations, using the specific VEGFR1 tyrosine site model. The g–i integrated responses and j–l phosphorylation amplitudes of all adapters were examined with altering g, j PLCγ concentration, h, k PI3K concentration, and i, l Src concentration, using the specific VEGFR1 tyrosine site model. Adapter concentrations were ranged between 10² and 10⁵ molecules/cell. The vertical gray dashed lines indicate the physiological adapter concentration in HUVECs (SI Table S1). Data are presented as mean ± standard deviation given by 4000 Monte Carlo simulations, described in the Supplementary Information