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. 2017 Dec 19;7:17855. doi: 10.1038/s41598-017-18140-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of AT on the antibody response to vaccination in MNP groups. (A) HAI presented as described in Fig. 3. Groups: R - RD (n = 6, black symbols), W - WD (n = 6, orange symbols), A - AT (n = 6, blue symbols). (B–D) Total vaccine-specific IgG, IgG1, and IgG2a, respectively. Filled and open circles connected with solid or broken lines represent immunized and naïve groups, respectively. “a” and “w” represent differences described in the legend for Fig. 3: p = 0.026 for “a” at day 14, p < 0.001 and p = 0.006 for “a” and “w” respectively at 28 on the panel B; p = 0.0026 for “a” on panel C. Detailed antibody responses of individual mice are shown in Supplemental Fig. 6.