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. 2017 Dec 19;7:17855. doi: 10.1038/s41598-017-18140-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MNP vaccination of AT-treated animals elicited higher antibody response than IM vaccination. (A) Comparison of the total vaccine-specific IgG at days 14 and 28 postvaccination in the IM (blue bar, replotted from Fig. 3) and MNP (patterned blue bar, replotted from Fig. 4) groups. (B) Comparison of HAI titers measured at days 14 and 28 postvaccination, same groups as on A. Individual data points for each mouse are shown in Supplemental Fig. 7.