Table 2.

Primers used for RT-PCR and real time PCR in gene expression analysis.

Gene	Primer sequence	Product size
GADPH	F: 5′ACC ACA GTC CAT GCC ATC AC 3′	450 bp
	R: 5′TCC ACC ACC CTG TTG CTG TA3′
Snail	F: 5′AAG CTT CCA TGG CGC GCT CTT TCC TCG TCA GGA AGC CC3′	795 bp
	R: 5′GGA TCC TCA GCG GGG ACA TCC TGA GCA GCC GGA CTC TTG3′
ZEB1	F: 5′TTC AGC ATC ACC AGG CAG TC3′	736 bp
	R: 5′GAG TGG AGG AGG CTG AGT AG3′
MMP9	F: 5′TGT ACC CTA TGT ACC GCT TCA CT3′	489 bp
	R: 5′AGA AGA AAA GCT TCT TGG AGA GC A3′
Fibronectin	F: 5′AAGGAG AAG ACCGGA CCA AT	314 bp
	R:GGC TTG ATG GTT CTC TGG AT
LEF1	F:TGGCA GCCCT ATTTC AGTTT	280 bp
	R:CAAAG GCTGT GCTTG CTTTT