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. 2017 Dec 19;7:17780. doi: 10.1038/s41598-017-17996-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of ten highly expressed lncRNAs and three lncRNAs that interacted with the FSHb gene. (A) Expression of up- and down-regulated lncRNAs in the mature and immature rat anterior pituitary. (B) Interaction network of lncRNAs and the FSHb gene. The green cycle represents FSHb genes, and the red triangle represents novel lncRNAs. (C–E) Expression level of three novel lncRNAs, MSTRG.80236.1, MSTRG.80236.2 and MSTRG.80236.3, in the mature and immature rat anterior pituitary. All experiments were repeated at least three times. The data are shown as the means ± SD. Statistical significance was analyzed by one-way ANOVA, p < 0.05 was considered significant, and differences are marked with the letters a and b.