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. 2017 Dec 19;7:17775. doi: 10.1038/s41598-017-18195-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Data from the validation flow cytometry assays. Panels (a–c) show representative flow cytometry blots from experiments for frequencies of Granzyme-B, γδ TCR (gdTCR), and CD94-expressing cytotoxic cells. Panels (d–f) present the results summary from the flow cytometry analyses with statistics. Each bar represents the mean and standard deviation of the data for each cell population. (d) Percent of intracellular or surface granzyme-B + cells in the pools of CD3+ CD8+ and CD56+ cell populations from 8–10 subjects with acute infectious mononucleosis (AIM) and 10–15 healthy controls (CTRL) in the secondary cohort. (e) Number of cytotoxic cells in 10,000 PBMCs from 10 patients with AIM and 10 healthy controls. (f) Percent of cytotoxic cells in a specific cell population from the same cohort of 10 AIM and 10 healthy control subjects. Mann-Whitney U test was performed and the significant difference was marked with *(p < 0.05), ***(p < 0.001) and ns for no significance (p > 0.2) between AIM vs. CTRL.