Figure 3.

Afh mutants do not exhibit food anticipatory activity. (a) Distribution of nose pokes, Average ± SEM, over 24 h in LD (n = 6 wild-type, n = 6 Afh). NP activity was quantified with 15 min time bins. Grey shaded regions indicate dark periods and yellow shaded regions indicate the time when food was available. Nose poking activity is averaged across 10 days during LD (a) and 7 days during DD (b) for the mutant mice (red lines) and wild-type littermate controls (blue lines). The inset show magnification of a selected time windows (5:00 to 10:00) for the LD (a₁) and DD (b₁). The black squares at the bottom of each graphs identified the intervals with significant genotype difference (p < 0.05, one-way Anova).