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. 2017 Dec 19;7:17765. doi: 10.1038/s41598-017-18130-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Afh mutants show disrupted sleep homeostasis. (a) Total sleep time in baseline (BS) and recovery (RC) period after sleep deprivation (SD) and in constant darkness (DD). (b) EEG delta power during NREM epochs is plotted for all phases. (c) Process S across all phases. Power density values for the entire BS, RC and DD phases are presented for NREM (d) and REM (e) epochs. All graphs are presented as mean ± SEM over either 1 hour intervals (a–c) or as frequencies (d,e). The black bars at the bottom of each graph indicate intervals where significant genotype differences were identified (n = 6 wild-types; n = 6 Afh mutants; P < 0.05, One-way ANOVA). The gray shadowed regions represent the dark phases. All graphs in DD are presented as CTs where data are rescaled according to subjective periodicities (see Methods).