Table 3.
DPA released and retained by spores after incubation on bSi and control wafers.
| Spores or cells analyzed | DPA - % of levels in starting spores1 | |||
|---|---|---|---|---|
| Spores/cells incubated on: | control wafers | bSi wafers | ||
| DPA: | released | retained | released | retained |
| B. subtilis wild-type spores | 2 | 100 | 1 | 100 |
| B. subtilis coatless spores | 1 | 97 | 2 | 96 |
| B. megaterium GR-less spores | 1 | 100 | 2 | 98 |
| B. cereus spores | 1 | 98 | 1 | 99 |
Spores were prepared and incubated on control or bSi wafers for 48 hr, spores harvested, and DPA in the starting and recovered spore pellets (retained DPA) and supernatant fluid obtained in spore recovery (released DPA) were determined in duplicate in two separate experiments, all as described in Methods. Values from duplicates differed by less than 6% from each other.
1Values are relative to values for the total DPA in untreated starting spores that were set at 100%.