Figure 5.

Improved growth of L. monocytogenes by addition of 1,2-PD. Growth curves of L. monocytogenes EGDe (□) and L. monocytogenes EGDe ΔpduD (○) in MM and 0.5% (w/v) yeast extract without glucose (a) or with 50 mM glucose (b) cultivated at 37 °C under anaerobic conditions. MM was supplemented with 10 mM 1,2-PD and 25 nM cobalamin (filled symbols) or not supplemented (open symbols). OD₆₀₀ was measured at the indicated intervals using Bioscreen C. Growth curves depict the calculated mean value of three independent biological experiments with technical duplicates, while error bars indicate the standard deviation.