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. 2017 Dec 19;7:17771. doi: 10.1038/s41598-017-17651-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RMCE strategy. The landing pad vector (LPV) including the GFP reporter and the PuroTK selection marker gene is integrated into the host genome by lentiviral transduction. The donor vector (DV_RMCE) includes a non-functional RFP reporter and the GOI expression unit (upper panel). Flp recombinase-mediated cassette exchange of the FRT-flanked DV_RMCE sequences results in the functional reconstitution of the RFP reporter gene along with the insertion of the GOI (middle panel). If desired, the fluorescent reporter can be selectively deleted via Cre recombination (lower panel). See Results section for vector features and abbreviations.