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. 2017 Dec 19;7:17771. doi: 10.1038/s41598-017-17651-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Side-by-side comparison of RMCE and HDR recombination frequencies. Both transgene integration systems targeting the same loci in cell clone CHO.S18 in a side-by-side comparison. The fluorescent marker protein distribution of unselected cell pools 9 days after transfection is shown. Below each plot the plasmids used for transient transfection are listed for both the RMCE and HDR approach. For details of the transfection reactions, see Methods section (summary table: mean ± standard deviation; n = 3).