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. 2017 Dec 19;7:17769. doi: 10.1038/s41598-017-18227-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Indirect staining of virus. Supernatants of chronically infected and uninfected cells labeled with (A) DiD, (B) DiI, (C) FM4-64, (D) DHPE-Rhodamine, and (E) Ceramide-BODIPY TR were analyzed by NFC. (F) Relative virus infectivity (transducing units (TU) per mL) and (G) counts of eGFP+ particles released from the supernatants of infected cells labeled with the different dyes. Infectivity and counts are presented as the percentage of total number of eGFP+ particles released from unstained virus-infected cells. Data depicts the mean with S.D. from three independent experiments. (H) Supernatants from DiD-stained infected and uninfected cells were treated with 0.05% Triton X-100.