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. 2017 Jun 14;34(12):2517–2531. doi: 10.1007/s11095-017-2190-7

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Distribution and functionality of endocytic compartments in ATI-like lung epithelia. Co-localisation of Caveolin-1 positive vesicles with (2a) EEA-1 immunopositive endosomes and (2b) lysosomes labelled by a 2 h/3 h pulse-chase internalization of dextran. CtxB internalized into ATI-like cells localizes to (2c) early endosomes after 10 min, (2d) a peri-nuclear compartment after 45 min. (2e) only a minor fraction of CtxB puncta localized to lysosomes following a 2 h/3 h pulse-chase co-internalisation of CtxB and 10 kDa dextran. Empty arrowheads in 2D highlight non-perinuclear puncta present within upstream compartments. (2f) TMR-labelled BSA accumulated in EEA-1 positive endosomes after a 10 min incubation. (2g–I) Primary ATI-like cells show a decrease in the density of PAMAM-OG staining density pattern that accompanies an increase in dendrimer generation from 1.5 to 5.5 after 30 min incubation. (j) a fraction of PAMAM 1.5 co-localised with dextran labelled lysosomes after a 2 h/3 h pulse-chase co-internalisation. Both PAMAM 3.5 (k) and PAMAM 5.5 (l) co-localise with BSA after 30 mins co-internalisation. Arrows in zoomed section highlight co-localised puncta. Scale bar: 5 μm.