Fig. 2.

Mutation and tagging effects. a Absolute chemical shift differences between WT and Lu³⁺-tagged mutant ntd-HSP90 resonances for ¹H (red) and ¹³C (blue) are plotted for each methyl group. The dotted lines mark significant changes, with 0.2 ppm for ¹³C and 0.02 for ¹H. b Location of methyl groups with unmatched resonances relative to the tag site. Distances are expressed in Ångström