Fig. 4.

Illustration of the peak pairing problem. The peaks for V172 and L48 are on the same correlation line in the spectrum of Yb³⁺ tagged ntd-HSP90, making the pairing to the peaks in the spectrum of Lu³⁺ tagged protein problematic. In this case, the observation that the V172 in the paramagnetic state is more intense than the L48 peak in the diamagnetic state, suggests that those two cannot be paired