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. 2017 Dec 19;86(1):e00522-17. doi: 10.1128/IAI.00522-17

FIG 2.

FIG 2

B. abortus induction of a fibrotic phenotype depends on PI3-kinase and lysosomal proteases. (A) Effect of wortmannin (wort) and E64 plus leupeptin (E64+leupeptin) in the inhibition of MMP-9 secretion induced by B. abortus infection by zymography 24 h postinfection. (B) wort and E64+leupeptin effect on the induction of collagen deposition induced by B. abortus infection, as determined by quantification of Sirius red staining 7 days after infection. (C) Effect of wort and E64+leupeptin on TGF-β1 secretion during B. abortus infection. Effect of rapamycin during B. abortus infection on MMP-9 secretion by zymography 24 h postinfection (D) and collagen deposition determined by quantification of Sirius red staining 7 days after infection (E). Data are given as the means ± SD from at least three individual experiments. *, P < 0.05; ***, P < 0.001 versus infected and untreated cells.