FIG 3.

Caspase-1 and ASC are required for inflammasome activation and IL-1β processing during M. pneumoniae infection. Cell culture supernatants from WT, caspase-1 KO, and ASC KO BMDMs infected with M. pneumoniae were assayed by ELISA for IL-1β (A) and IL-6 (B) levels. (C) Immunoblot analysis for pro-IL-1β in cell lysates of WT, ASC KO, and caspase-1 KO BMDMs infected with M. pneumoniae. The mock-infected control is designated M. β-Actin served as the loading control. Data are representative of two independent experiments with similar results. **, P < 0.01. h.p.i., hours postinfection.