FIG 4.

NF-κB signaling is required for expression of proinflammatory genes and inflammasome activation during M. pneumoniae infection. Cell culture supernatants from M. pneumoniae-infected WT BMDMs treated with the NF-κB inhibitor BAY11-7082 were assayed by ELISA for IL-1β (A) and IL-6 (B) levels. (C) Immunoblot analysis of IL-1β p17 and caspase-1 p10 in supernatants and pro-IL-1β and procaspase-1 in cell lysates of vehicle (DMSO-treated) or BAY11-7082-treated WT BMDMs infected with M. pneumoniae. The mock-infected control is designated M. β-Actin served as the loading control. Data are representative of three independent experiments with similar results. *, P < 0.05; **, P < 0.01; ***, P < 0.001. h.p.i., hours postinfection.