FIG 5.

MyD88 signaling is required for expression of proinflammatory genes and inflammasome activation during M. pneumoniae infection. Cell culture supernatants from WT and MyD88 KO BMDMs infected with M. pneumoniae were assayed by ELISA for IL-1β (A) and IL-6 (B) levels. (C) Immunoblot analysis of IL-1β p17 and caspase-1 p10 in supernatants and pro-IL-1β and procaspase-1 in cell lysates of WT and MyD88 KO BMDMs infected with M. pneumoniae. The mock-infected control is designated M. β-Actin served as the loading control. Data are representative of three independent experiments with similar results. ***, P < 0.001. h.p.i., hours postinfection.