FIG 5.

Deletion of OG1RF_11272 in the ΔireK mutant background is sufficient to elicit the suppressor mutant phenotype. (A) The cholate and lysozyme resistance of the WT E. faecalis strain (OG1RF) and the ΔireK (CK119), ΔireK ΔOG1RF_11271 (IB21), ΔireK ΔOG1RF_11272 (IB22), and ΔireK ΔOG1RF_11271 ΔOG1RF_11272 (IB23) mutants was determined. The reported MICs represent the median values from three independent biological replicates. (B) CPRG hydrolysis was measured for the strains listed in panel A. The reported measurements represent averages from three independent cultures, and error bars represent standard deviations. Statistical significance was evaluated by t test. ****, P < 0.0001. (C) Groups of mice (5 per group) were colonized with the ΔireK (CK119), ΔireK ΔOG1RF_11271 (IB21), or ΔireK ΔOG1RF_11272 (IB22) mutant. Colonization loads were determined by enumerating the enterococci in feces by culture on rifampin-supplemented BHI agar. Dashed line, the limit of detection. Symbols for mice with undetectable colonization levels were omitted, and instead, the number of mice for which colonization was not detected (ND)/total number of mice tested is shown underneath the dotted line.