FIG 7.

Restriction of OG1RF_11271 and OG1RF_11272 actions require IreK's kinase activity. (A) The cholate and lysozyme resistance of a strain with an ireK K41R mutation (BL102) carrying an empty vector as well as an ireK K41R ΔOG1RF_11271 ΔOG1RF_11272 mutant (IB36) carrying an empty vector or complemented with OG1RF_11271-OG1RF_11272 was determined. The reported MICs represent the median values from three independent biological replicates. (B) CPRG hydrolysis was measured for the strains listed in panel A. The reported measurements represent averages from three independent cultures, and error bars represent standard deviations. Statistical significance was evaluated by t test. ****, P < 0.0001. (C) Proposed model for IreK and OG1RF_11271/OG1RF_11272 modulation of cell envelope integrity, antimicrobial resistance, and colonization. (Inset) A nonscaled depiction of the protein domain architecture for OG1RF_11271 and OG1RF_11272.