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. 2017 Dec 19;86(1):e00724-17. doi: 10.1128/IAI.00724-17

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Copyright © 2017 Burtnick et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 3 — Functional analysis of antibody responses raised against CPS-CRM197, Hcp1, and TssM. C57BL/6 mice (n = 6 per group) were immunized on days 0, 21, and 35 with adjuvant only (Alhydrogel/CpG), conjugate only, Hcp1 only, TssM only, conjugate plus Hcp1, and conjugate plus TssM. Immune serum samples were collected on day 42. B. pseudomallei K96243 was incubated with (a) medium only (no-serum control), pooled HI adjuvant (Alhydrogel/CpG)-only immune serum, pooled HI conjugate-only immune serum, pooled HI Hcp1-only immune serum, pooled HI conjugate plus Hcp1-immune serum, or (b) medium only (no-serum control), pooled HI adjuvant (Alhydrogel/CpG)-only immune serum, pooled HI conjugate-only immune serum, pooled HI TssM-only immune serum, or pooled HI conjugate plus TssM immune serum. Following incubation for 1 h, opsonized bacteria were added to RAW 264.7 murine macrophage monolayers. Uptake was quantitated at 3 h postinfection. Reported values represent mean results ± standard deviations for three individual assays conducted in triplicate. Figures are representative of at least three independent experiments conducted on different days. ns, not significant; *, P < 0.05.