TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Descriptiona	Reference or source
Escherichia coli strain
TOP10	Lab strain for cloning and protein expression	Life Technologies
Burkholderia pseudomallei strains
RR2683	OPS-deficient derivative of select agent excluded strain Bp82; ΔpurM ΔrmlD	61
K96243	Wild type; clinical isolate from Thailand	62
Plasmids
pBAD/HisA	Arabinose inducible, 6× His tag expression vector; Apr	Life Technologies
pBADBmhcp1-6HisF	pBAD/HisA containing B. mallei hcp1 (BMAA0742) with an N-terminal His tag	33
pMB1000	pUC57Kan containing B. pseudomallei tssM (BPSS1512) corresponding to amino acids 191–474 in which Cys codon (TGC) at position 102 in TssM changed to Gly codon (GGC)	This study (GenScript)
pMB1001	pBAD/HisA containing B. pseudomallei tssM (BPSS1512) corresponding to amino acids 191–474 in which Cys codon (TGC) at position 102 in TssM changed to Gly codon (GGC); N-terminal His tag	This study

^aAp^r, ampicillin resistance.