FIG 7.

ERAD is impeded in cells ectopically expressing Ank4. (A) Western blots of lysates from HEK-293T cells stably expressing YFP-TCRα transfected to also express Flag-tagged BAP, Ank4, or Ank4ΔF-box were screened with antibodies that recognize YFP, β-actin, and the Flag tag. Vertical lines between the lanes indicate that irrelevant lanes of the blot were removed. (B) Densitometry was performed on blots resulting from three separate experiments. Statistically significant values are indicated: *, P < 0.05; n.s., not significant.