TABLE 2.
Ank4 candidate interacting partners identified via yeast two-hybrid analysis
| Candidate | Name and function | PBSa | No. of positive clones/total XX |
|---|---|---|---|
| SKP1 | S-phase kinase-associated protein 1; binds F-box in the SCF1 ubiquitin ligase complex | A | 22/197 |
| Bat3 | HLA-B-associated transcript 3 (also known as Bag6 and Scythe); ERAD chaperone, regulator of p53, HSP70, and apoptosis | C | 2/197 |
| HSP90AB1 | Heat shock protein 90-kDa alpha (cytosolic), class B member 1; role in protein folding and signal transduction | A | 28/197 |
| HIF1AN | Hypoxia-inducible factor 1 alpha subunit inhibitor; functions as oxygen sensor | A | 6/197 |
| VCPIP | Valosin-containing protein p97/p47 complete interacting protein 1; deubiquitinating enzyme, involved in postmitosis Golgi complex reassembly | C | 2/197 |
a
The predicted biological score (PBS) was calculated for each candidate protein to assess the reliability of each interaction, ranging from the highest probability of specificity (score of A) to the lowest probability of specificity (score of E) between two proteins.