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Intracellular Pi concentrations in S. aureus decrease during Pi limitation. (A) Growth of wild-type S. aureus in PFM9, pH 7.4, supplemented with various concentrations of Pi, measured by OD600. n = 3; error bars indicate SEMs and are frequently smaller than the symbols. (B) Intracellular Pi levels normalized to protein concentration in wild-type S. aureus grown in PFM9 supplemented with the indicated concentrations of Pi. n ≥ 2; error bars indicate SEMs. *, P < 0.05 via unpaired t test.
