TABLE 2.

Doubling times of P_i transporter mutants in tryptic soy broth^a

Strain description	Doubling time, mean ± SD (min)
WT	33.0 ± 1.7
ΔpstSCAB	31.8 ± 0.6
ΔpitA	32.8 ± 1.0
ΔnptA	30.3 ± 0.6*
ΔpstSCAB ΔpitA	32.0 ± 0.3
ΔpstSCAB ΔnptA	31.8 ± 1.0
ΔnptA ΔpitA	37.5 ± 1.5*
WT pEV	32.8 ± 0.6
ΔpitA pEV	32.5 ± 0.6
ΔnptA ΔpitA pEV	38.2 ± 1.1*
ΔnptA ΔpitA pnptA	32.2 ± 0.6
WT pPTet-empty	39.5 ± 0.7
ΔnptA pPTet-empty	42.7 ± 2.8
ΔnptA ΔpitA pPTet-empty	48.3 ± 2.0*
ΔnptA ΔpitA pPTet-pitA	40.8 ± 1.5

^a*, P < 0.05 compared to wild type via one-way ANOVA with Sidak's posttest. WT, wild type; pEV, empty vector.