TABLE 2.

Strains, derived isogenic isolates, and plasmids used in the present work

Strain or plasmid	Relevant featuresa	Reference or source
Salmonella strains
3246	S. Dublin wild-type, bstA-positive strain	22
4/74	S. Typhimurium wild-type, virulent reference strain	23
KP1274	Escherichia coli restriction-deficient strain	38
ΔbstA	S. Dublin 3246 lacking bstA, Aprr	This work
3246-C	S. Dublin ΔbstA complemented with bstA by pACY177 + bstA::pAHF4, Aprr Knr	This work
3246/pACY177	S. Dublin 3246 containing pACY177	This work
invH201::TnphoA	4/74 lacking invH, Knr	35
4/74 ΔssaV	4/74 lacking ssaV, SPI2-T3SS defect, Knr	This work
Plasmids
pACY177	Cloning vector, Apr Knr	39
pAHF4	pACY177 expressing bstA, Knr	10
pKD46	Plasmid with bacteriophage λ red recombinase expressed from an arabinose-inducible promoter, Apr	32
pUO9090	Aprr	Unpublished
pKD4	Knr	32

^aApr^r, Kn^r, and Ap^r, apramycin, kanamycin, and ampicillin resistant, respectively.