FIG 1.

Targeted amino acid modifications within the PfMSP8 carrier domain of rPfMSP1/8 improve the yield of monomeric rPfMSP1/8(CΔS). (A) Schematic of the original pET28a-PfMSP1/8 construct (top) depicting two cysteine residues (red asterisks) within the PfMSP8 domain that were mutated to serine residues via site-directed mutagenesis, resulting in the construct pET28a-PfMSP1/8(CΔS) (bottom). (B) Lysates of E. coli transformed with pET28a-PfMSP1/8(CΔS), harvested before (T₀) or 3 h after (T₃) induction, were separated by SDS-PAGE (10% gel) under both reducing and nonreducing conditions, followed by Coomassie blue staining. (C) Immunoblot analysis of T₀ and T₃ lysates probed with rabbit anti-PfMSP1/8 IgG. (D and E) Purified rPfMSP1/8 (original) (lane 1) and rPfMSP1/8(CΔS) (modified) (lane 2) were separated by SDS-PAGE (10% gel) under nonreducing conditions, followed by Coomassie blue staining (3 μg/lane) (D) or immunoblot analysis (50 ng/lane) (E) of samples probed with rabbit anti-PfMSP1/8 IgG.