FIG 8.

Immunization with rPfs25 or rPfs25/8(CΔS) elicits Pfs25-specific antibodies that recognize native Pfs25. (A) Recognition of native Pfs25 by pooled rabbit antisera generated against rPfs25, rPfs25/8(CΔS), or the adjuvant control was assessed by an immunofluorescence assay on activated, fixed macrogametes. Samples probed with MAb 4B7 were used as a positive control. Bound antibodies were detected with FITC-conjugated secondary IgG. (B) The purified macrogamete lysate was separated by SDS-PAGE (12% gels) under nonreducing conditions, followed by an immunoblot assay using pooled sera from rabbits immunized with rPfs25 (lane 1), rPfs25/8(CΔS) (lane 2), or the adjuvant alone (lane 3).