TABLE 1.
Immunization with rPfs25 and rPfs25/8(CΔS) elicits antibodies with similar transmission-reducing activitiesa
| Immunization group | Rabbit | IgG level (μg/ml) | Transmission-reducing activity (expt 1 and 2) |
|||
|---|---|---|---|---|---|---|
| % inhibition | 95% CI (low) | 95% CI (high) | P value (vs alum control) | |||
| Alum control | 1 | 938 | 0 | |||
| rPfs25 | 2 | 182 | 51.3 | 14.2 | 72.5 | 0.015 |
| 3 | 156 | 48.4 | 9.9 | 71.2 | 0.015 | |
| 4 | 356 | 62.4 | 31.3 | 78.6 | 0.002 | |
| 5 | 221 | 55.4 | 19.9 | 74.3 | 0.008 | |
| rPfs25/8(CΔS) | 6 | 360 | 53.1 | 16.2 | 73.4 | 0.013 |
| 7 | 218 | 53.1 | 16.5 | 73.5 | 0.018 | |
| 8 | 579 | 54.2 | 19.8 | 73.7 | 0.005 | |
| 9 | 699 | 47.1 | 4.9 | 69.9 | 0.030 | |
a
Total IgG from individual rabbits immunized with rPfs25 and rPfs25/8(CΔS) was purified and tested in a standard membrane feeding assay at low total IgG concentrations, as indicated, normalized to an anti-rPfs25 titer of 2,000. The best estimates and 95% confidence intervals (CIs) of percent inhibitions and P values were calculated from two independent assays and each experiment with 20 mosquitoes per IgG sample. The P value for the rPfs25 and rPfs25/8(CΔS) groups was 0.63.