FIG 3.

Exosialidase activities of C. perfringens culture supernatants. Seven C. perfringens strains (Table 1) were grown in either TH broth (A), for vegetative growth, or DS sporulation medium (B) for 14 h at 37°C. Following this incubation, cell-free culture supernatants were prepared by centrifugation, followed by the filtration of the supernatants to remove any bacterial cells. The total exosialidase activity in the culture supernatants was then determined spectrophotometrically, as described previously (23). The experiment was performed in triplicate, and the means are depicted. Error bars indicate the standard errors.