FIG 2.

Chlamydia infection results in the production of ECM proteins. Monolayers of murine oviduct epithelial cells were infected with C. trachomatis serovar D, and after 48 h, immunofluorescence staining of the cells for collagen types I and III was performed on infected and noninfected monolayers by standard procedures. Images were acquired with a 20× objective on a Nikon fluorescence microscope with the same microscope settings and exposure time. The representative slides are from at least 3 repeated experiments showing similar results.