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. 2017 Dec 15;8:1840. doi: 10.3389/fimmu.2017.01840

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Copyright © 2017 Jiang, Chen, Zhang, Zhang, Ye, Liu, Yu, Wei, Ren and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interleukin-6 (IL-6)-induced suppression of suppressor of cytokine signaling 3 (SOCS3) in myeloid-derived suppressor cells (MDSCs) was determined at both the mRNA and protein levels. (A) RT-PCR result showed mRNA level of SOCS1 was increased and SOCS3 was significantly decreased in MDSCs compared to in both CD33⁺ and CD14⁺ controls; an undetectable level of SOCS2 mRNA was observed in both MDSCs and controls (n = 20). (B) The mRNA levels of SOCS1–3 between MDSCs from IL-6^high tissues (MDSC^IL-6h) and MDSCs from IL-6^low tissues (MDSC^IL-6l) were compared. The mRNA level of SOCS1 was increased in MDSC^IL-6h (P = 0.0459), while the mRNA level of SOCS3 was decreased in MDSC^IL-6h compared to in MDSC^IL-6l (P = 0.0089). Linear regression analysis demonstrated that IL-6 expression was not correlated with SOCS1 (R² = 0.09071, P = 0.2102), but demonstrated a significant negative correlation with SOCS3 expression (R² = 0.2205, P = 0.0367) (n = 20). (C) Then, we detected mRNA levels of SOCS1–3 in induced MDSCs in vitro. The results were consistent with those observed in primary MDSCs, where the mRNA level of SOCS1 was increased, while that of SOCS3 was decreased in MDSCs compared to in the controls (n = 5). (D) The whole cellular lysates were collected for western blot analysis. SOCS1 protein was notably increased, but SOCS3 protein was dramatically decreased in MDSCs as compared with those in CD33⁺ negative controls and iDC positive controls (n = 5). (E) RT-PCR show that Ab-treated MDSCs displayed slightly decreased mRNA level in SOCS1 (P = 0.0917), but significantly higher mRNA levels in SOCS3 compared to in Ab-untreated MDSCs (P = 0.0117) (n = 5). (F) Consistent results were confirmed at the protein level (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001.

Interleukin-6 (IL-6)-induced suppression of suppressor of cytokine signaling 3 (SOCS3) in myeloid-derived suppressor cells (MDSCs) was determined at both the mRNA and protein levels. (A) RT-PCR result showed mRNA level of SOCS1 was increased and SOCS3 was significantly decreased in MDSCs compared to in both CD33⁺ and CD14⁺ controls; an undetectable level of SOCS2 mRNA was observed in both MDSCs and controls (n = 20). (B) The mRNA levels of SOCS1–3 between MDSCs from IL-6^high tissues (MDSC^IL-6h) and MDSCs from IL-6^low tissues (MDSC^IL-6l) were compared. The mRNA level of SOCS1 was increased in MDSC^IL-6h (P = 0.0459), while the mRNA level of SOCS3 was decreased in MDSC^IL-6h compared to in MDSC^IL-6l (P = 0.0089). Linear regression analysis demonstrated that IL-6 expression was not correlated with SOCS1 (R² = 0.09071, P = 0.2102), but demonstrated a significant negative correlation with SOCS3 expression (R² = 0.2205, P = 0.0367) (n = 20). (C) Then, we detected mRNA levels of SOCS1–3 in induced MDSCs in vitro. The results were consistent with those observed in primary MDSCs, where the mRNA level of SOCS1 was increased, while that of SOCS3 was decreased in MDSCs compared to in the controls (n = 5). (D) The whole cellular lysates were collected for western blot analysis. SOCS1 protein was notably increased, but SOCS3 protein was dramatically decreased in MDSCs as compared with those in CD33⁺ negative controls and iDC positive controls (n = 5). (E) RT-PCR show that Ab-treated MDSCs displayed slightly decreased mRNA level in SOCS1 (P = 0.0917), but significantly higher mRNA levels in SOCS3 compared to in Ab-untreated MDSCs (P = 0.0117) (n = 5). (F) Consistent results were confirmed at the protein level (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001.