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. 2017 Dec 5;2017:4896310. doi: 10.1155/2017/4896310

Figure 3.

Figure 3

Immunofluorescence staining of TAMs reveals colocalization of TAMs and PFC nanoemulsions (scale bar: 50 μm). (a) DiI on PFC nanoemulsions. (b) FITC on F4/80 antibodies. (c) Merged images of DAPI, DiI, and FITC staining showing colocalization of TAMs and PFC nanoemulsions (yellow); TAMs that are not labeled with PFC nanoemulsions are also observed (green). TAM, tumor-associated macrophage; PFC, perfluorocarbon; DiI, 1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate; FITC, fluorescein isothiocyanate; DAPI, 4′,6-diamidino-2-phenylindole.