FIG 3 .

Differential expression of opp genes throughout the enzootic cycle. Data represent results of qRT-PCR analysis of transcripts from (A) fed larvae, (B) flat nymphs, (C) fed nymphs, and mammalian-host-adapted spirochetes cultivated in dialysis membrane chambers (DMCs) (D). Note that for spirochetes from unfed ticks and DMCs, the y axis is expanded because of the lower transcript copy numbers. Values represent averages of results from experiments performed with three biological samples, each done in quadruplicate. Error bars indicate standard errors of the means. P values for pairwise comparisons, determined using a two-tailed t test, are provided in Table S1.