FIG 1 .
SLC6A14 is an important modulator of l-arginine uptake in airway epithelium. l-[2,3-3H]arginine was used to monitor the intracellular uptake of arginine from the apical surfaces of airway epithelium harvested from mice or cultured with a human cell line (Calu-3). (A) l-[2,3-3H]arginine uptake, expressed in counts per minute per concentration of whole tissue lysate, significantly decreased following genetic knockout of Slc6a14 (Slc6a14−/y) or SLC6A14 inhibition with α-MT in wild-type (Slc6a14+/y) mice. Data points represent independent biological replicates ± the standard deviation. Asterisks indicate a P value of <0.05 (one-way analysis of variance with Tukey’s multiple-comparison test). (B) Significant reduction in l-[2,3-3H]arginine uptake upon inhibition of SLC6A14 with α-MT in Calu-3 airway epithelial monolayers. Data points represent three independent biological replicates (each consisting of four technical replicates) ± the standard deviation. Asterisks indicate a P value of <0.05 (two-tailed unpaired t test).