Recombination activities along chromosome III in cells sporulated at 14°C, 30°C, or 37°C. The y axis shows the hybridization values (log2 of the ratio of Spo11-enriched sample divided by control DNA sample) for each oligonucleotide on the microarray ranked from 0 (lowest) to 1 (highest). The x axis shows the Saccharomyces Genome Database (SGD) coordinates on chromosome III in kilobases. Blue, black, and red lines indicate the recombination activities in cells sporulated at 14°C, 30°C, and 37°C, respectively. The HIS4 hot spot on the left arm has its highest activity at 14°C, intermediate activity at 30°C, and very little activity at 37°C. In contrast, the activity of the IMG1-ARE1 hot spot on the right arm is unaffected by temperature.