FIG 5 .

Microarray and Southern blot analysis of two recombination hot spots. (A) Recombination activities of oligonucleotides located near the HIS4 and BIK1 genes as determined by microarray analysis. The activity of this hot spot is strongest in cells sporulated at 14°C (blue line). (B) Recombination activities of oligonucleotides (microarray analysis) located near the SPR3 and ERG25 genes. The activity of this hot spot is strongest in cells sporulated at 37°C (red line). (C) HIS4 hot spot. (D) ERG25 hot spot. Southern blot analysis of genomic DNA isolated from control DNA (nonsporulated) and of cells sporulated at 14°C, 30°C, or 37°C was performed. Genomic DNA was digested with BglII (C) or BamHI (D). Following gel electrophoresis and DNA transfer to nylon membranes, the samples were hybridized to PCR-generated probes (details are given in Materials and Methods). The slow-migrating band in each lane is the expected size for genomic fragments without a meiosis-specific DSB, and the fast-migrating band is the expected size for the hot spot-associated DSB. (E) HIS4. (F) ERG25. We show the ratios of the meiosis-specific DNA fragment relative to the intact DNA fragment as determined with ImageJ software.