Table 1: Identification of the specificity and phenotype of IFNγ- secreting T cells by intracellular staining. Splenocytes were collected 1 week after the last immunization. Individual peptides (2 |jg/ml final) derived from pools were used to stimulate splenocytes ex vivo for 1 hour prior to an overnight incubation with brefeldin A (2 jg/ml final). Cells were then stained with anti-CD3, -CD4 and -CD8 labeled antibodies. After fixation and permeabilization, intracellular IFNγ- detection was performed. ND: Not determined due to the lack of cells
| NASVAC | DNAs | ||||||||||||||||
| Pools | A2-DR1 | HBs-A2-DR1 | A2-DR1 | HBs-A2-DR1 | |||||||||||||
| Peptide | ICS | Peptide | ICS | Peptide | ICS | Peptide | ICS | ||||||||||
| C1-55 | 21-35 | CD4 | 21-35 | ND | ND | CD4 CD8 | 16-30 | CD8 | |||||||||
| C46-95 | 56-70 | CD4 | 56-70 | ND | ND | CD4 CD8 | ND | ND | |||||||||
| 61-75 | CD4 | 61-75 | |||||||||||||||
| C86-140 | 111-125 | CD4 | 111-125 | CD4 | ND | CD4 CD8 | |||||||||||
| 115-130 | CD4 | 115-130 | CD4 | ||||||||||||||
| PreS2 | 164-128 | CD4 | |||||||||||||||
| S164-218 | 200-214 ad | CD4 | 200-214 ad | CD4 | 164-178 | CD8 | 164-178 | ND | |||||||||
| 170-181 | CD8 | 200-214 ad | CD8 | ||||||||||||||
| 200-214 ad | CD8 | 204-212 ad | CD8 | ||||||||||||||
| 204-212 ad | CD8 | ||||||||||||||||
| S338-389 | 348-357 | CD8 |