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. 2017 Dec 13;8:902. doi: 10.3389/fphar.2017.00902

FIGURE 2.

FIGURE 2

Downregulation of HDAC2 and HDAC5 increases the sensitivity to tamoxifen and restores the sensitivity to estrogen deprivation in MCF7-TamC3 cells. (A,B) MCF7, MCF7-TamC3, and ZR-75-1 cells were transfected with scramble siRNA, HDAC2 siRNA, or HDAC5 siRNA. Cell viability and cytotoxicity was assessed by the MTT assay (96 h post-treatment) and LDH assay (5 days post-treatment), respectively. (C) Left panel: ZR-75-1 cells were cultured under either estrogen-containing (full medium) or estrogen-deprived conditions for 96 h. Cell viability was assessed by the MTT assay. Right panel: ZR-75-1 cells were pre-transfected with either scramble siRNA or HDAC2 siRNA for 24 h and subsequently cultured under either estrogen-containing or estrogen-deprived conditions for 96 h. Cell viability was assessed by the MTT assay. (D) MCF7-TamC3 cells were pre-transfected with scramble siRNA, HDAC2 siRNA, or HDAC5 siRNA for 24 h and co-treated with or without tamoxifen for 72 h. Cell viability was assessed by the MTT assay. (E) MCF7-TamC3 cells were pre-transfected with scramble siRNA, HDAC2 siRNA, or HDAC5 siRNA for 24 h and subsequently cultured under either estrogen-containing or estrogen-deprived conditions for 72 h. Cell viability was assessed by the MTT assay. “∗”, “∗∗”, and “∗∗∗” denote a statistical significance (P < 0.05, P < 0.01, and P < 0.001, respectively) between the testing groups. “N.S.” denotes no statistical significance between the testing groups.